Product details
0.9% Sodium Chloride Injection (isotonic saline) is the standard neutral-pH, physiological-osmolarity reconstitution diluent for peptides that are soluble and stable at neutral pH. The 0.9% NaCl concentration gives a solution isotonic with blood plasma (≈ 300 mOsm/L), which minimizes osmotic stress when reconstituted material is introduced into cell-culture systems or in vivo models. It is the workhorse diluent for the majority of synthetic-peptide reconstitution workflows without aggregation tendency — in contrast to proteins like IGF-1 LR3 that require the acidic-pH acetic-acid diluent instead. It is supplied as a characterized ancillary diluent rather than a research-target molecule.
Peptuno supplies 0.9% Saline in sterile vial formats prepared from pharmaceutical-grade sodium chloride and Water for Injection. The release packet documents sterility, bacterial endotoxin (LAL), osmolality, and pH per pharmacopeia standards; the diluent is supplied to the USP monograph by default, with EP / BP / JP equivalents available on request for buyers whose destination market requires a specific monograph reference.
Regulatory note: Isotonic reconstitution diluent; supplied for Research Use Only. Verify sterility, pyrogen (LAL), osmolality, and injectable-grade compliance with the destination market's requirements before use.
FAQ
- Why is 0.9% saline isotonic, and why does isotonicity matter for reconstitution?
- The 0.9% NaCl concentration (≈ 300 mOsm/L) matches the osmolality of blood plasma and extracellular fluid, so it drives no net water movement across cell membranes — avoiding the hemolysis of hypotonic preparations or the cell-shrinkage of hypertonic ones. For peptide reconstitution this produces working solutions physiologically compatible with both in vivo administration and most cell-culture buffers. Peptides requiring acidic pH use the 0.6% acetic-acid diluent instead; single-use preservative-free preparations use SWFI.
- Which pharmacopeia specification applies to the saline diluent?
- 0.9% Sodium Chloride Injection is monographed in USP, EP, BP, and JP. The specifications cover identity (sodium and chloride content), sterility, bacterial endotoxin (LAL), pH, particulate matter, and osmolality. Peptuno supplies to the USP monograph by default; buyers in EU, UK, or APAC markets needing a local-pharmacopeia spec should specify at quote stage so the documentation references the appropriate monograph.
- Which peptides should not be reconstituted in saline?
- Proteins and peptides that aggregate or precipitate at neutral pH — IGF-1 LR3 and unmodified MGF are the common examples — should use the 0.6% acetic-acid diluent rather than saline. The peptide's recommended diluent is noted on its COA; saline is the default for the large majority of synthetic peptides that are soluble and stable at neutral pH.
Certificate of Analysis (COA)
The per-lot COA for this product will appear here.
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